85-6048-26　GRAP2 cDNA 10ug　551984

特徴

• GRAP2, also known as GADS, is a member of the GRB2/Sem5/Drk family. This member is an adaptor-like protein involved in leukocyte-specific protein-tyrosine kinase signaling. Like its related family member, GRB2-related adaptor protein (GRAP), this protein contains an SH2 domain flanked by two SH3 domains. This protein interacts with other proteins, such as GRB2-associated binding protein 1 (GAB1) and the SLP-76 leukocyte protein (LCP2), through its SH3 domains.
• Vector Description:This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.
• cDNA Size:993bp
• Preparation before Usage:1. Centrifuge at 7000rpm for 1 minute
• 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA
• Each tube contains approximately 10ug of lyophilized plasmid.
• Nucleotide Sequence:ATGGAAGCTG TTGCCAAGTT TGATTTCACT GCTTCAGGTG AGGATGAACT GAGCTTTCAC ACTGGAGATG TTTTGAAGAT TTTAAGTAAC CAAGAGGAGT GGTTTAAGGC GGAGCTTGGG AGCCAGGAAG GATATGTGCC CAAGAATTTC ATAGACATCC AGTTTCCCAA ATGGTTTCAC GAAGGCCTCT CTCGACACCA GGCAGAGAAC TTACTCATGG GCAAGGAGGT TGGCTTCTTC ATCATCCGGG CCAGCCAGAG CTCCCCAGGG GACTTCTCCA TCTCTGTCAG GCATGAGGAT GACGTTCAAC ACTTCAAGGT CATGCGAGAC AACAAGGGTA ATTACTTTCT GTGGACTGAG AAGTTTCCAT CCCTAAATAA GCTGGTAGAC TACTACAGGA CAAATTCCAT CTCCAGACAG AAGCAGATCT TCCTTAGAGA CAGAACCCGA GAAGACCAGG GTCACCGGGG CAACAGCCTG GACCGGAGGT CCCAGGGAGG CCCACACCTC AGTGGGGCTG TGGGAGAAGA AATCCGACCT TCGATGAACC GGAAGCTGTC GGATCACCCC CCGACCCTTC CCCTGCAGCA GCACCAGCAC CAGCCACAGC CTCCGCAATA TGCCCCAGCG CCCCAGCAGC TGCAGCAGCC CCCACAGCAG CGATATCTGC AGCACCACCA TTTCCACCAG GAACGCCGAG GAGGCAGCCT TGACATAAAT GATGGGCATT GTGGCACCGG CTTGGGCAGT GAAATGAATG CGGCCCTCAT GCATCGGAGA CACACAGACC CAGTGCAGCT CCAGGCGGCA GGGCGAGTGC GGTGGGCCCG GGCGCTGTAT GACTTTGAGG CCCTGGAGGA TGACGAGCTG GGGTTCCACA GCGGGGAGGT GGTGGAGGTC CTGGATAGCT CCAACCCATC CTGGTGGACC GGCCGCCTGC ACAACAAGCT GGGCCTCTTC CCTGCCAACT ACGTGGCACC CATGACCCGA TAA
• Translation Sequence:MEAVAKFDFT ASGEDELSFH TGDVLKILSN QEEWFKAELG SQEGYVPKNF IDIQFPKWFH EGLSRHQAEN LLMGKEVGFF IIRASQSSPG DFSISVRHED DVQHFKVMRD NKGNYFLWTE KFPSLNKLVD YYRTNSISRQ KQIFLRDRTR EDQGHRGNSL DRRSQGGPHL SGAVGEEIRP SMNRKLSDHP PTLPLQQHQH QPQPPQYAPA PQQLQQPPQQ RYLQHHHFHQ ERRGGSLDIN DGHCGTGLGS EMNAALMHRR HTDPVQLQAA GRVRWARALY DFEALEDDEL GFHSGEVVEV LDSSNPSWWT GRLHNKLGLF PANYVAPMTR
• Storage and Stability:Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.