85-6047-89　ESRRA cDNA 10ug　551951

特徴

• ESRRA encoded by this gene is a nuclear receptor that is closely related to the estrogen receptor. ESRRA acts as a site-specific transcription regulator and has been also shown to interact with estrogen and the transcripton factor TFIIB by direct protein-protein contact. The binding and regulatory activities of this protein have been demonstrated in the regulation of a variety of genes including lactoferrin, osteopontin, medium-chain acyl coenzyme A dehydrogenase (MCAD) and thyroid hormone receptor genes. A processed pseudogene of ESRRA is located on chromosome 13q12. 1. Alternatively spliced transcript variants have been found for this gene.
• Vector Description:This shuttle vector contains the complete ORF. It is inseted BamH I to Hind III. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.
• cDNA Size:1272bp
• Preparation before Usage:1. Centrifuge at 7000rpm for 1 minute
• 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA
• Each tube contains approximately 10ug of lyophilized plasmid.
• Nucleotide Sequence:ATGTCCAGCC AGGTGGTGGG CATTGAGCCT CTCTACATCA AGGCAGAGCC GGCCAGCCCT GACAGTCCAA AGGGTTCCTC GGAGACAGAG ACCGAGCCTC CTGTGGCCCT GGCCCCTGGT CCAGCTCCCA CTCGCTGCCT CCCAGGCCAC AAGGAAGAGG AGGATGGGGA GGGGGCTGGG CCTGGCGAGC AGGGCGGTGG GAAGCTGGTG CTCAGCTCCC TGCCCAAGCG CCTCTGCCTG GTCTGTGGGG ACGTGGCCTC CGGCTACCAC TATGGTGTGG CATCCTGTGA GGCCTGCAAA GCCTTCTTCA AGAGGACCAT CCAGGGGAGC ATCGAGTACA GCTGTCCGGC CTCCAACGAG TGTGAGATCA CCAAGCGGAG ACGCAAGGCC TGCCAGGCCT GCCGCTTCAC CAAGTGCCTG CGGGTGGGCA TGCTCAAGGA GGGAGTGCGC CTGGACCGCG TCCGGGGTGG GCGGCAGAAG TACAAGCGGC GGCCGGAGGT GGACCCACTG CCCTTCCCGG GCCCCTTCCC TGCTGGGCCC CTGGCAGTCG CTGGAGGCCC CCGGAAGACA GCAGCCCCAG TGAATGCACT GGTGTCTCAT CTGCTGGTGG TTGAGCCTGA GAAGCTCTAT GCCATGCCTG ACCCCGCAGG CCCTGATGGG CACCTCCCAG CCGTGGCTAC CCTCTGTGAC CTCTTTGACC GAGAGATTGT GGTCACCATC AGCTGGGCCA AGAGCATCCC AGGCTTCTCA TCGCTGTCGC TGTCTGACCA GATGTCAGTA CTGCAGAGCG TGTGGATGGA GGTGCTGGTG CTGGGTGTGG CCCAGCGCTC ACTGCCACTG CAGGATGAGC TGGCCTTCGC TGAGGACTTA GTCCTGGATG AAGAGGGGGC ACGGGCAGCT GGCCTGGGGG AACTGGGGGC TGCCCTGCTG CAACTAGTGC GGCGGCTGCA GGCCCTGCGG CTGGAGCGAG AGGAGTATGT TCTACTAAAG GCCTTGGCCC TTGCCAATTC AGACTCTGTG CACATCGAAG ATGCCGAGGC TGTGGAGCAG CTGCGAGAAG CTCTGCACGA GGCCCTGCTG GAGTATGAAG CCGGCCGGGC TGGCCCCGGA GGGGGTGCTG AGCGGCGGCG GGCGGGCAGG CTGCTGCTCA CGCTACCGCT CCTCCGCCAG ACAGCGGGCA AAGTGCTGGC CCATTTCTAT GGGGTGAAGC TGGAGGGCAA GGTGCCCATG CACAAGCTGT TCTTGGAGAT GCTCGAGGCC ATGATGGACT GA
• Translation Sequence:MSSQVVGIEP LYIKAEPASP DSPKGSSETE TEPPVALAPG PAPTRCLPGH KEEEDGEGAG PGEQGGGKLV LSSLPKRLCL VCGDVASGYH YGVASCEACK AFFKRTIQGS IEYSCPASNE CEITKRRRKA CQACRFTKCL RVGMLKEGVR LDRVRGGRQK YKRRPEVDPL PFPGPFPAGP LAVAGGPRKT AAPVNALVSH LLVVEPEKLY AMPDPAGPDG HLPAVATLCD LFDREIVVTI SWAKSIPGFS SLSLSDQMSV LQSVWMEVLV LGVAQRSLPL QDELAFAEDL VLDEEGARAA GLGELGAALL QLVRRLQALR LEREEYVLLK ALALANSDSV HIEDAEAVEQ LREALHEALL EYEAGRAGPG GGAERRRAGR LLLTLPLLRQ TAGKVLAHFY GVKLEGKVPM HKLFLEMLEA MMD
• Storage and Stability:Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.