85-6047-85　ELK1 cDNA 10ug　551947

特徴

• ELK1 is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum response element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. This gene produces multiple isoforms by using alternative translational start codons and by alternative splicing. Related pseudogenes have been identified on chromosomes 7 and 14.
• Vector Description:This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.
• cDNA Size:1287bp
• Preparation before Usage:1. Centrifuge at 7000rpm for 1 minute
• 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA
• Each tube contains approximately 10ug of lyophilized plasmid.
• Nucleotide Sequence:ATGGACCCAT CTGTGACGCT GTGGCAGTTT CTGCTGCAGC TGCTGAGAGA GCAAGGCAAT GGCCACATCA TCTCCTGGAC TTCACGGGAT GGTGGTGAAT TCAAGCTGGT GGATGCAGAG GAGGTGGCCC GGCTGTGGGG GCTACGCAAG AACAAGACCA ACATGAATTA CGACAAGCTC AGCCGGGCCT TGCGGTACTA CTATGACAAG AACATCATCC GCAAGGTGAG CGGCCAGAAG TTCGTCTACA AGTTTGTGTC CTACCCTGAG GTCGCAGGGT GCTCCACTGA GGACTGCCCG CCCCAGCCAG AGGTGTCTGT TACCTCCACC ATGCCAAATG TGGCCCCTGC TGCTATACAT GCCGCCCCAG GGGACACTGT CTCTGGAAAG CCAGGCACAC CCAAGGGTGC AGGAATGGCA GGCCCAGGCG GTTTGGCACG CAGCAGCCGG AACGAGTACA TGCGCTCGGG CCTCTATTCC ACCTTCACCA TCCAGTCTCT GCAGCCGCAG CCACCCCCTC ATCCTCGGCC TGCTGTGGTG CTCCCCAGTG CAGCTCCTGC AGGGGCAGCA GCGCCCCCCT CGGGGAGCAG GAGCACCAGT CCAAGCCCCT TGGAGGCCTG TCTGGAGGCT GAAGAGGCCG GCTTGCCTCT GCAGGTCATC CTGACCCCGC CCGAGGCCCC AAACCTGAAA TCGGAAGAGC TTAATGTGGA GCCGGGTTTG GGCCGGGCTT TGCCCCCAGA AGTGAAAGTA GAAGGGCCCA AGGAAGAGTT GGAAGTTGCG GGGGAGAGAG GGTTTGTGCC AGAAACCACC AAGGCCGAGC CAGAAGTCCC TCCACAGGAG GGCGTGCCAG CCCGGCTGCC CGCGGTTGTT ATGGACACCG CAGGGCAGGC GGGCGGCCAT GCGGCTTCCA GCCCTGAGAT CTCCCAGCCG CAGAAGGGCC GGAAGCCCCG GGACCTAGAG CTTCCACTCA GCCCGAGCCT GCTAGGTGGG CCGGGACCCG AACGGACCCC AGGATCGGGA AGTGGCTCCG GCCTCCAGGC TCCGGGGCCG GCGCTGACCC CATCCCTGCT TCCTACGCAT ACATTGACCC CGGTGCTGCT GACACCCAGC TCGCTGCCTC CTAGCATTCA CTTCTGGAGC ACCCTGAGTC CCATTGCGCC CCGTAGCCCG GCCAAGCTCT CCTTCCAGTT TCCATCCAGT GGCAGCGCCC AGGTGCACAT CCCTTCTATC AGCGTGGATG GCCTCTCGAC CCCCGTGGTG CTCTCCCCAG GGCCCCAGAA GCCATGA
• Translation Sequence:MDPSVTLWQF LLQLLREQGN GHIISWTSRD GGEFKLVDAE EVARLWGLRK NKTNMNYDKL SRALRYYYDK NIIRKVSGQK FVYKFVSYPE VAGCSTEDCP PQPEVSVTST MPNVAPAAIH AAPGDTVSGK PGTPKGAGMA GPGGLARSSR NEYMRSGLYS TFTIQSLQPQ PPPHPRPAVV LPSAAPAGAA APPSGSRSTS PSPLEACLEA EEAGLPLQVI LTPPEAPNLK SEELNVEPGL GRALPPEVKV EGPKEELEVA GERGFVPETT KAEPEVPPQE GVPARLPAVV MDTAGQAGGH AASSPEISQP QKGRKPRDLE LPLSPSLLGG PGPERTPGSG SGSGLQAPGP ALTPSLLPTH TLTPVLLTPS SLPPSIHFWS TLSPIAPRSP AKLSFQFPSS GSAQVHIPSI SVDGLSTPVV LSPGPQKP
• Storage and Stability:Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.