85-6047-81　EIF2B1 cDNA 10ug　551943

特徴

• EIF2B1 encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter.
• Vector Description:This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.
• cDNA Size:918bp
• Preparation before Usage:1. Centrifuge at 7000rpm for 1 minute
• 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA
• Each tube contains approximately 10ug of lyophilized plasmid.
• Nucleotide Sequence:ATGGACGACAAGGAGTTAATTGAATACTTTAAGTCTCAGATGAAAGAAGATCCTGACATGGCCTCAGCAGTGGCTGCCATCCGGACGTTGCTGGAGTTCTTGAAGAGAGATAAAGGGGAGACAATCCAGGGTCTGAGGGCGAATCTCACCAGTGCCATAGAAACCCTGTGTGGTGTGGACTCCTCTGTGGCAGTGTCCTCTGGCGGGGAGCTCTTCCTCCGCTTCATCAGTCTTGCCTCCCTGGAATACTCCGATTACTCCAAATGTAAAAAGATCATGATTGAGCGGGGAGAACTTTTTCTCAGGAGAATATCACTGTCAAGAAACAAAATTGCAGATCTGTGCCATACTTTCATCAAAGATGGAGCGACAATATTGACTCACGCCTACTCCAGAGTGGTCCTGAGAGTCCTGGAAGCAGCCGTGGCGGCCAAGAAGCGATTTAGTGTATACGTCACAGAGTCACAGCCTGATTTGTCAGGTAAGAAAATGGCCAAAGCCCTCTGCCACCTCAACGTCCCTGTCACTGTGGTGCTAGATGCTGCTGTCGGCTACATCATGGAGAAAGCAGATCTTGTCATAGTTGGTGCTGAAGGAGTTGTTGAAAACGGAGGAATTATTAACAAGATTGGAACCAACCAGATGGCTGTGTGTGCCAAAGCACAGAACAAACCTTTCTATGTGGTTGCAGAAAGTTTCAAGTTTGTCCGGCTCTTTCCACTAAACCAGCAAGACGTCCCAGATAAGTTTAAGTATAAGGCAGACACTCTCAAGGTCGCGCAGACTGGACAAGACCTCAAAGAGGAGCATCCGTGGGTCGACTACACTGCCCCTTCCTTAATCACTCTGCTGTTTACAGACCTGGGCGTGCTGACACCCTCAGCAGTCAGCGATGAGCTCATCAAGCTCTATCTGTAA
• Translation Sequence:MDDKELIEYF KSQMKEDPDM ASAVAAIRTL LEFLKRDKGE TIQGLRANLT SAIETLCGVDSSVAVSSGGE LFLRFISLAS LEYSDYSKCK KIMIERGELF LRRISLSRNK IADLCHTFIKDGATILTHAY SRVVLRVLEA AVAAKKRFSV YVTESQPDLS GKKMAKALCH LNVPVTVVLDAAVGYIMEKA DLVIVGAEGV VENGGIINKI GTNQMAVCAK AQNKPFYVVA ESFKFVRLFPLNQQDVPDKF KYKADTLKVA QTGQDLKEEH PWVDYTAPSL ITLLFTDLGV LTPSAVSDELIKLYL
• Storage and Stability:Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.