Auxiliary enzyme of beta-oxidation. Participates in the degradation of unsaturated fatty enoyl-CoA esters having double bonds in both even- and odd-numbered positions in peroxisome. Catalyzes the NADP-dependent reduction of 2, 4-dienoyl-CoA to yield trans-3-enoyl-CoA. Has activity towards short and medium chain 2, 4-dienoyl-CoAs, but also towards 2, 4, 7, 10, 13, 16, 19-docosaheptaenoyl-CoA, suggesting that it does not constitute a rate limiting step in the peroxisomal degradation of docosahexaenoic acid.
Vector Description:This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.
cDNA Size:879bp
Preparation before Usage:1. Centrifuge at 7000rpm for 1 minute
2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA
Each tube contains approximately 10ug of lyophilized plasmid.
Storage and Stability:Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
