85-6047-45　DCAF7 cDNA 10ug　551908

特徴

• DCAF7, also known as DDB1-and CuL4-associated factor, involved in craniofacial development. This protein acts upstream of the EDN1 pathway and is required for formation of the upper jaw equivalent, the palatoquadrate. The activity required for EDN1 pathway function differs between the first and second arches. This protein associates with DIAPH1 and controls GLI1 transcriptional activity. This protein could be involved in normal and disease skin development.
• Vector Description:This shuttle vector contains the complete ORF. It is inseted Nde I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.
• cDNA Size:1029bp
• Preparation before Usage:1. Centrifuge at 7000rpm for 1 minute
• 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA
• Each tube contains approximately 10ug of lyophilized plasmid.
• Nucleotide Sequence:ATGTCCCTGC ACGGCAAACG GAAGGAGATC TACAAGTATG AAGCGCCCTG GACAGTCTAC GCGATGAACT GGAGTGTGCG GCCCGATAAG CGCTTTCGCT TGGCGCTGGG CAGCTTCGTG GAGGAGTACA ACAACAAGGT TCAGCTTGTT GGTTTAGATG AGGAGAGTTC AGAGTTTATT TGCAGAAACA CCTTTGACCA CCCATACCCC ACCACAAAGC TCATGTGGAT CCCTGACACA AAAGGCGTCT ATCCAGACCT ACTGGCAACA AGCGGTGACT ATCTCCGTGT GTGGAGGGTT GGTGAAACAG AGACCAGGCT GGAGTGTTTG CTAAACAATA ATAAGAACTC TGATTTCTGT GCTCCCCTGA CCTCCTTTGA CTGGAATGAG GTGGATCCTT ATCTTTTAGG TACCTCAAGC ATTGATACGA CATGCACCAT CTGGGGGCTG GAGACAGGGC AGGTGTTAGG GCGAGTGAAT CTCGTGTCTG GCCACGTGAA GACCCAGCTG ATCGCCCATG ACAAAGAGGT CTATGATATT GCATTTAGCC GGGCCGGGGG TGGCAGGGAC ATGTTTGCCT CTGTGGGTGC TGATGGCTCG GTGCGGATGT TTGACCTCCG CCATCTAGAA CACAGCACCA TCATTTACGA AGACCCACAG CATCACCCAC TGCTTCGCCT CTGCTGGAAC AAGCAGGACC CTAACTACCT GGCCACCATG GCCATGGATG GAATGGAGGT GGTGATTCTA GATGTCCGGG TTCCCTGCAC ACCTGTCGCC AGGTTAAACA ACCATCGAGC ATGTGTCAAT GGCATTGCTT GGGCCCCACA TTCATCCTGC CACATCTGCA CTGCAGCGGA TGACCACCAG GCTCTCATCT GGGACATCCA GCAAATGCCC CGAGCCATTG AGGACCCTAT CCTGGCCTAC ACAGCTGAAG GAGAGATCAA CAATGTGCAG TGGGCATCAA CTCAGCCCGA CTGGATCGCC ATCTGCTACA ACAACTGCCT GGAGATACTC AGAGTGTAG
• Translation Sequence:MSLHGKRKEI YKYEAPWTVY AMNWSVRPDK RFRLALGSFV EEYNNKVQLV GLDEESSEFI CRNTFDHPYP TTKLMWIPDT KGVYPDLLAT SGDYLRVWRV GETETRLECL LNNNKNSDFC APLTSFDWNE VDPYLLGTSS IDTTCTIWGL ETGQVLGRVN LVSGHVKTQL IAHDKEVYDI AFSRAGGGRD MFASVGADGS VRMFDLRHLE HSTIIYEDPQ HHPLLRLCWN KQDPNYLATM AMDGMEVVIL DVRVPCTPVA RLNNHRACVN GIAWAPHSSC HICTAADDHQ ALIWDIQQMP RAIEDPILAY TAEGEINNVQ WASTQPDWIA ICYNNCLEIL RV
• Storage and Stability:Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.