jetBLUEX the protein staining solution, is a proprietary nanoparticle-based, ready-to-use protein staining solution for SDS-PAGE gels. It is formulated for fast and sensitive protein detection without any destaining required, features generally observed for the use of InstantBlue-level protein stains. In the absence of hazardous substances such as methanol and acetic acid, jetBLUEX is considered to be safe and environmentally friendly. jetBLUEX is also compatible with mass spectrophotometry.
Quality Control:The quality of this product is tested on a lot-to-lot basis to ensure consistent quality.
Required Material and Equipment not Provided:SDS-PAGEDistilled waterContainer: box for gel staining Shaker: orbital or rocking shaker
Protocol:1. After SDS-PAGE is finished, remove the gel from the cassette and incubate the gel in distilled water with three changes of fresh water, 10 minutes each time
2. Decant water and submerge the gel in proper amount of jetBLUEX dye, enough to cover the gel. Mildly agitate the staining box temperature for 10 to 30 minutes at room temperature
3. Remove the dye solution carefully. Wash the membrane with large amount of distilled water with shaking.