Cytosolic Protamine Kinase (cPK) is a distinct insulin-stimulated protein kinase. This enzyme phosphorylates protein synthesis initiation factor eIF4E at serine 209 and threonine 210. It also phosphorylates hnRNP protein A1, and several other cellular proteins. cPK is itself regulated by reversible phosphorylation. It is activated by phosphorylation by MBPK-1 and MBPK-2. By contrast, PP2A1, PP2A2 and PP2AC dephosphorylate and inactivate the kinase.
Source:Bovine Kidney
Molecular Weight:~45kD
Biological Activity:~17,000 units/mg with protamine sulfate as substrate.
Unit Definition:One unit is the amount of cPK that incorporates 1nmol of phosphate into protamine sulfate/min.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ng
Source Antigen：Bovine kidney
Grade：Purified
Purity：≥90% (SDS-PAGE)
Form：Supplied as a liquid in 50mM Tris-HCl, pH 7.0, 14mM β-mercaptoethanol, 1mM benzamidine, 0.1mM phenylmethanesulfonyl fluoride, 1mM EDTA, 50% glycerol.
