85-6044-29　［取扱停止］Coronavirus OC43 Lysate, Human 100ug　544715

特徴

• The coronaviruses are a family of related RNA viruses within the order Nidovirales. They contain a positive-sense, single-stranded, 26-32kb RNA genome protected by a nucleocapsid of helical symmetry. Their viral capsids are surrounded by a lipid envelope, which is interrupted by trimeric Spike proteins that project from the capsid surface. Human coronavirus OC43 (HCoV-OC43) is a betacoronavirus and is known to infect humans and cattle. HCoV-OC43 infects cells by binding the N-acetyl-9-O-acetlyneuraminic acid receptor (Neu5Ac) (Li, 2016)
• HCoV-OC43 infection results in a mild cold. If infects its hosts by gaining entry into the respiratory epithelium, using its Hemagglutinin (HE) and Spike (S) glycoproteins. Like the other mild cold-causing human coronaviruses, HCoV-229E, HCoV-NL63 and HCoV-HKU1, HCoV-OC43 has a worldwide distribution, with seasonal surges occurring in the winter months (Wat et al., 2004). Like the other cold-causing coronaviruses, reinfection with HCoV-OC43 is common. The reason for this is not yet clearly defined but may be due to a weak antibody response (Raoult et al., 2020).
• During coronavirus replication, numerous proteins are syntheses by the host cell, including Nucleoprotein, Spike, Envelope, Membrane, non-structural proteins and accessory proteins. Of particular importance in the development of serological diagnostics and vaccines are the Spike proteins, which are easily recognized by host antibodies given their exposure to the external environment. The Spike protein’s receptor binding domain (RBD) is also responsible for binding host cell-surface receptors to mediate cell entry, making it the target of neutralising antibodies. Nucleoprotein is also a popular target for countermeasures, given its high sequence conservation.
• Source:Human coronavirus OC43 viral lysate expressed in HCT-8 cells
• Applications:Suitable for use in Immunoassay Development, Western Blot, Dot Blot and other Protein-based Assays. Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Inactivation:Heat-inactivated and tested for absence of viral growth in validated tissue culture infectivity assays.
• Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.