SARS-CoV-2 is a respiratory virus which causes coronavirus disease 2019 (COVID-19); it spreads primarily through contact with an infected person through respiratory droplets generated when a person coughs or sneezes, or through droplets of saliva or discharge from the nose. The incubation period is believed to range from 2-11 days. Infection with SARS-CoV-2 can cause mild symptoms including a runny nose, sore throat, cough, and fever. However, it can be more severe for some people and can lead to pneumonia or breathing difficulties. The elderly, and people with pre-existing medical conditions (such as, diabetes and heart disease) appear to be more vulnerable to becoming severely ill with the virus (WHO, 2020).
Coronaviruses encode five structural proteins in their genomes; namely, the Spike (S), Membrane (M), Envelope (E) glycoproteins, Hemagglutinin Esterase (HE) and Nucleoprotein (N), (Figure 2). All envelope proteins and N protein is present in all virions but HE is only present in some beta coronaviruses (Tok and Tatar, 2017). There are three main groups of coronaviruses: alpha, beta, and gamma. Nucleoproteins, also known as nucleocapsid proteins, are phosphoproteins that are capable of binding to helix and have flexible structure of viral genomic RNA. It plays an important role in virion structure, replication and transcription of coronaviruses, as it localizes in both the replication/ transcriptional region of the coronaviruses and the ERGIC region where the virus is collected. It packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. It also plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. There is structural similarity between porcine reproductive and respiratory syndrome virus (PRRSV), a member of Arteriviridae, suggesting a common origin for Coronaviridae and Arteriviridae nucleoproteins (Yu et al., 2006).
Recombinant protein corresponding to aa1-419 from full length SARS-CoV-2 Nucleocapsid phosphoprotein, strain Wuhan-Hu-1, fused to 6xHis-Tag at N-terminal, expressed in E. coli.
Molecular Weight:~47kD
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Source Antigen：Recombinant, E. coli
Grade：Purified
Purity：≥90%
Form：Supplied as a liquid in 20mM sodium phosphate, 25mM potassium carbonate, pH 10.0, 150mM sodium chloride.
