Recombinant lectin protein with a Hexa-His Tag from Burkholderia cenocepacia Man2, expressed in E. coli.
Purity:≥95% (SDS PAGE analysis using Coomassie Blue detection and gel Permeation Chromatography)
Form:Supplied as a liquid in PBS, pH 7.2.
Specificity:Terminal α-mannose
Molecular Weight:15,163D (Monomer), Protein is a dimer under physiological conditions (PBS, pH 7.2)
Usage Guidelines:
RPL activity requires metal ions. All buffers must be supplemented with 1mM CaCl2, MgCl2 and MnCl2
Recommended dilution to desired working concenration (≤20ug/ml) in TBS, pH 7.6
Following dilution with TBS and supplemented with metal ions, the RPL should be incubated for a minimum of 30 minutes prior to use to allow the metal complexation.
Theoretical Ext. Coeffocoent [E0.1%]:0.559
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
