The D-Dimer (D2D) BioAssay™ ELISA Kit Kit is a competitive inhibition immunoassay for the in vitro quantitative measurement of D2D in rat plasma.
Detection Range:123.5-10,000pg/ml
Sensitivity:47.7pg/ml
Intra-Assay CV:<10%
Inter-Assay CV:<12%
Kit Components:*152478A: Microtiter Plate, 1x96 wells. Pre-coated; ready to use
*152478B: Standard, 2x1vial 152478C: Standard Diluent, 1x20ml*152478D: Detection Reagent A, 1x120ul *152478E: Detection Reagent B, 1x120ul 152478F: Assay Diluent A, 1x12ml152478G: Assay Diluent B, 1x12ml152478H: TMB Substrate, 1x9ml*152478J: Positive Control 13820pg/ml, 1x1vial (Lyophilized)152478L: Wash Buffer, 30X, 1x20ml
Storage and Stability:Store *152478A, *152478B, *152478D, *152478E and *152478J at -20°C. After reconstitution of *152478J, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Notes on 152478: Positive Control:Intended Use:The positive control is from a rat serum sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.
Characteristics:The positive control vial contains D2D in rat serum (lyophilized powder). Note: The rat source material used in this product are from healthy animal of SPF standard (specific pathogen-free animals).
Expected Values:13820pg/ml
Usage:Dissolve with 150ul standard diluent and let stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 50ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: Upon receipt store Positive Control vial at -20°C. After reconstitution, store Positive Control vial at 4°C and use within 5 days
Assay Procedure Summary:1. Prepare all reagents, samples and standards
2. Add 50ul standard or sample to each well
3. Add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C
6. Aspirate and wash 5 times
7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37°C
8. Add 50ul Stop Solution. Read at 450nm immediately.
