IDO catalyzes the first and rate-limiting step in the main pathway of human tryptophan catabolism, the kynurenine pathway. Proinflammatory mediators, such as endotoxin and IFN-gamma induce the expression of IDO in several tissues. IDO-dependent suppression of T cell responses might function as natural immunoregulatory mechanism. Physiological IDO activity has been implicated in T cell tolerance to tumors, dysfunctional selftolerance in non-obese diabetic (NOD) mice, and as a protective negative regulator in autoimmune disorders.
Source:Recombinant protein corresponding to aa2-403 from human IDO1, fused to His-Tag at N-terminal, expressed in E. coli.
Molecular Weight:~46kD
Assay Conditions:IDO1 activity was confirmed using the IDO1 Inhibitor Screening BioAssay™ Kit and following the protocol provided with the kit.
Applications:Suitable for use in the study of enzyme kinetics, substrate specificity, and screening inhibitors. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Source Antigen：Recombinant, E. coli
Grade：Purified
Purity：≥90%
Form：Supplied as a liquid in 40mM Tris, pH 8, 110mM sodium chloride, 2.2mM potassium chloride, 20% glycerol.
