Source:Recombinant protein corresponding to aa18-286 from human CRTAM, fused to the Fc region of human IgG1 and with a C-terminal Avi-Tag, expressed in HEK293 cells.
Applications:Suitable for use as an antagonist to block PD-L1 signaling and binding to PD-1 in biochemical assays. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Assay Conditions:Anti-PD-L1-Biotin was titrated from 0-50nM with a 2-fold dilution series in presence of 100 nM PD-L1-FLAG and incubated for 1 hour at RT. Binding was detected with Anti-FLAG-Eu and Streptavidin APC.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant, HEK293 cells
Grade：Purified
Purity：≥86%
Form：Supplied as a liquid in PBS, pH 7.4, 20% glycerol. Labeled with Biotin.
