Source:Recombinant protein corresponding to aa18-286 from human CRTA, fused to C-terminal Avi-Tag fused to the Fc region of Human IgG1, expressed in HEK293 cells.
Applications:Suitable for use as a positive control for Western Blot. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Assay Conditions:Anti-PD-L1-Biotin was titrated from 0-50nM with a 2-fold dilution series in presence of 100 nM PD-L1-FLAG and incubated for 1 hour at RT. Binding was detected with Anti-FLAG-Eu and Streptavidin APC.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Recombinant, HEK293 cells
Grade：Purified
Purity：≥90%
Form：Supplied as a liquid in PBS, pH 7.4, 20% glycerol.
