Source:Recombinant protein corresponding to aa27-547 from human CD73, fused to Avi-His-Tag at C-terminal, expressed in HEK293 cells.
Molecular Weight:~61kD
Applications:Suitable for the use in study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested.
Assay Conditions:Assay was done in 50ul assay buffer containing 25mM Tris, pH 7.4, 5mM MgCl2 and 100uM AMP for 15 min at 37°C. Phosphate standard solution was run side-by-side for product quantification.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:50ug
Source Antigen:Recombinant, HEK293 cells
Grade:Purified
Purity:≥90%
Form:Supplied as a liquid in PBS, pH 7.4, 20% glycerol. Labeled with Biotin.