Source:Recombinant protein corresponding to aa1-54 from human BCMA, fused at the C-terminus to the Fc portion of Human IgG1 and with a C-terminal Avi-Tag, expressed in a HEK293 cells.
Molecular Weight:~34kD
Applications:Other applications not tested.
Assay Conditions:Reaction done according to BCMA:APRIL[B] BioAssay™ kit protocol. Buffer is PBS + 0.1% BSA. Blocking buffer is Superblock + 0.05% Tween-20. Coat protein (BCMA) was added overnight at 4 deg 100ng/well. Various amounts of biotinylated ligand (APRIL[B]) (0-20ng) added for 2h at RT. Strep-HRP added for 1h at RT.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Source Antigen：Recombinant, HEK293 cells
Grade：Purified
Purity：≥90%
Form：Supplied as a liquid in PBS, pH 7.4, 20% glycerol.
