Bacteriophage MS2 is a non-enveloped, single-stranded RNA virus that infects “male” Escherichia coli. It is part of the Levivirus genus which is 1 of 2 genera present in the Leviviridae family. The bacteriophages in this family infect their hosts through adsorption to the bacterial pili. MS2’s lack of an envelope renders it resistant to many chemical disinfectants and gives MS2 the ability to endure a range of environmental stresses including changes in temperature. The small MS2 3,569bp genome codes for 4 proteins: assembly, lysis, coat and RNA replicase. The MS2 genome was the first genome to be sequenced fully. The MS2 coat protein (CP) in particular has two known functions: to form the phage coat and to inhibit the translation of the phage replicase gene. It achieves the latter by binding a specific RNA structure. The MS2 phage has 6 main life cycle stages, namely: Adsorption to the cell, Translation of the phage RNA, Replication of the Phage RNA, Synthesis of the A protein, Particle assembly and Cell lysis. Amongst other things, MS2 is both harmless to humans and easy to grow, making it a great model organism for many macromolecular processes. Therefore fast and accurate detection with real time PCR is extremely beneficial.
Kit Usage:The Primerdesign genesig Kit for Bacteriophage MS2 (Bacteriophage MS2) genomes is designed for the in vitro quantification of Bacteriophage MS2 genomes. The kit is designed to have a broad detection profile. Specifically, the primers represent 100% homology with over 95% of the NCBI database reference sequences available at the time of design.
Bacteriophage MS2 Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens. A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
Clinical Relevance:The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.
Suitable Sample Material:All kinds of sample material suited for PCR amplification can be used. Please ensure the samples are suitable in terms of purity, concentration, and RNA/DNA integrity.
Dynamic Range of Test:Under optimal PCR conditions genesig Bacteriophage MS2 detection kits have very high priming efficiencies of >95% and can detect less than 100 copies of target template.
Kit Components: Bacteriophage MS2 specific primer/probe mix (BROWN)Once resuspended the kits should remain at -20ºC until ready to use.
Bacteriophage MS2 positive control template (RED lid)
Internal extraction control RNA (BLUE lid)
RNase/DNase free water (WHITE lid)
Template preparation buffer (YELLOW lid)
54 x genesig® q16 reaction tubes
Reagents and Equipment to be Supplied by User:q16 instrumentgenesig® Easy Extraction KitThis kit is designed to work well with all processes that yield high quality RNA and DNA but the genesig Easy extraction method is recommended for ease of use
genesig® Lab-In-A-BoxThe genesig Lab-In-A-Box contains all of the pipettes, tips and racks that you will need to use a genesig Easy kit. Alternatively if you already have these components and equipment these can be used instead.
Storage and Stability:This lyophilized kit is stable at room temperature but should be stored at -20ºC on arrival. Once the lyophilized components have been resuspended they should not be exposed to temperatures above -20°C for longer than 30 minutes at a time and unnecessary repeated freeze/thawing should be avoided. The kit is stable for six months from the date of resuspension under these circumstances. Primerdesign does not recommend using the kit after the expiry date stated on the pack.
