The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Recombinant protein corresponding to Val11-Arg682 from 2019-nCoV Spike S1, fused with an Fc-Tag and 6×His-Tag at C-terminal, expressed in HEK293 cells.
Biological Activity:Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human ACE2 at 2ug/mL (100uL/well) can bind Recombinant 2019-nCoV Spike S1-TEVS-hFc-His with a linear range of 78-82.5ng/ml.
Storage and Stability:Lyophilized and reconstituted products may be stored at -20°C. Stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Recombinant, HEK293 cells
Grade：Highly Purified
Purity：≥95% (SDS-PAGE). Endotoxin: <1EU/ug (LAL)
Form：Supplied as a lyophilized powder from a 0.22um filtered solution of PBS, pH 7.4. Reconstitute to 0.1-0.5mg/ml in sterile ddH2O.
