85-6038-92　［取扱停止］Plasmid miniPREP BioSeparation™ Kit 100Tests　544087

特徴

• The Plasmid miniPREP BioSeparation™ Kit provides a fast, simple, and cost-effective method for isolation of plasmid DNA from cultured Gram positive and Gram negative bacterial cells. The design is based on alkaline lysis of bacterial cells followed by binding of DNA onto the glass fiber matrix of the spin column in the presence of highly concentrated salts. Plasmid DNA purified with this kit is suitable for a variety of routine applications including restriction enzyme digestion, sequencing, library screening, in vitro translation, transfection of robust cells, ligation and transformation. The entire procedure can be completed within 15-20 minutes.
• Features:Fastest Procedure: Completed within 15-20 minsSample: up to 4ml bacterial cellsYield: up to 30ug of plasmid
• Kit Components:Buffer S1, 25mlBuffer S2, 25mlBuffer S3, 35mlBuffer W1, 45mlBuffer W2 (Add ethanol), 15ml (60ml)Buffer E, 10mlRNase A (50mg/ml), 50ulPM Columns, 100pcsCollection Tubes, 100pcs
• Required Materials:Ethanol (96∼100%)Microcentrifuge tubes
• Buffer Preparation:Add the provided RNase A solution to the Buffer S1, mix, and store at 2~8°C
• Add 60ml of ethanol (96~100%) to the Buffer W2 before use.