The Plasmid midiPREP BioSeparation™ Kit provides a fast, simple, and cost-effective plasmid midiprep method for isolating the plasmid DNA from the cultured bacterial cells. The Plasmid midiPREP BioSeparation™ Kit is based on the alkaline lysis of bacterial cells, followed by binding DNA onto the glass fiber matrix of the spin column in the presence of high salt. Phenol extraction and ethanol precipitation are not required, and the high-quality plasmid DNA is eluted in a small volume of the Tris buffer (included in each kit) or water (pH is between 7.0 and 8.5). The plasmid DNA purified with the Plasmid midiPREP BioSeparation™ Kit is suitable for a variety of routine applications, including the restriction enzyme digestion, sequencing, library screening, in vitro translation, transfection of robust cells, ligation, and transformation. The entire procedure can be completed within 40 minutes.
Sample:Up to 100ml bacterial cells
Yield:Up to 250ug of plasmid
Endotoxin Value:<0.003EU/ug
Applications:The purified plasmid DNA can be immediately used in any downstream molecular biology application
Features:Safe: Eliminates the use of phenol, chloroform, ethidium bromide, and cesium chloride, thus minimizing the exposure to and disposal of hazardous materials
Time saving: Complete the process in less than 40 minutes.
Kit Components:Buffer M1, 85mlBuffer M2, 85mlBuffer M3, 125mlBuffer W1, 125ml, 40mlBuffer W2, 25ml X 2*Add 100 ml of the ethanol (96~100%) to each bottle of the Buffer W2 before use
Buffer E, 50mlRNase A (50mg/ml), 200ulMD Column, 20 pcs
