85-6038-90　［取扱停止］Plasmid maxiPREP BioSeparation™ Kit 10Tests　544085

特徴

• The Plasmid maxiPREP BioSeparation™ Kit provides a fast, simple, and cost-effective plasmid maxiprep method for isolating the plasmid DNA from the cultured bacterial cells. The Plasmid maxiPREP BioSeparation™ Kit is based on the alkaline lysis of bacterial cells, followed by binding DNA onto the glass fiber matrix of the spin column in the presence of high salt. Phenol extraction and ethanol precipitation are not required, and the high-quality plasmid DNA is eluted in a small volume of the Tris buffer (included in each kit) or water (pH is between 7.0 and 8.5). The plasmid DNA purified with the Plasmid maxiPREP BioSeparation™ Kit is suitable for a variety of routine applications, including the restriction enzyme digestion, sequencing, library screening, in vitro translation, transfection of robust cells, ligation, and transformation. The entire procedure can be completed within 40 minutes.
• Sample:Up to 200ml bacterial cells
• Yield:Up to 850ug of plasmid
• Endotoxin Value:<0.003EU/ug
• Features:Safe: Eliminates the use of phenol, chloroform, ethidium bromide, and cesium chloride, thus minimizing the exposure to and disposal of hazardous materials
• Time saving: Complete the process in less than 40 minutes.
• Applications:* Transfecttion * Microinjection * Sequencing * PCR * Restriction enzyme digestion
• Kit Components:Buffer M1, 85mlBuffer M2, 85mlBuffer M3, 125mlBuffer W1, 105mlBuffer W2, 25ml*Add 100ml of ethanol prior to initial use
• Buffer E, 30mlRNase A (50mg/ml), 200ulMD Column, 10 pcs