The PCR Clean-Up & Gel Extraction BioSeparation™ Kit provides a cost-effective system for the fast and easy isolation of the DNA fragments from PCR reactions, agarose gels, or enzymatic reactions. The DNA fragments (100bp~10Kb) in the special buffers are bound by the glass fiber matrix of the spin column while contaminants pass through the column. Impurities are efficiently washed away, and the pure DNA is eluted with the Tris buffer or water without phenol extraction or alcohol precipitation. The DNA purified with the kits is suitable for any subsequent application, such as ligation and transformation, sequencing, restriction enzyme digestion, labeling, PCR, in vitro transcription, or microinjection. The entire procedure can be completed within 15~20 minutes.
Features:Fastest Procedure: Completed within 30minsSample: up to 100ul of PCR Product or 300mg of Agarose GelRecovery: up to 95%
Kit Components:Buffer B: 60mlBuffer W1: 45mlBuffer W2: 15ml (Add 60 ml of the ethanol (96~100%) to the Buffer W2 before use)Buffer E: 10mlPG Columns: 100 pcsCollection Tubes: 100 pcs
