The Virus Nucleic Acid Isolation Kit provides a fast, simple, and cost-effective method for the isolation of viral DNA/RNA from cell-free samples such as serum, plasma, body fluids and the supernatant of virus-infected cell cultures. Its unique buffer system will efficiently lyse cells and degrade protein, allowing for the nucleic acid to be easily bound by the glass fiber matrix of the column. Contaminants such as salts, metabolites and soluble macromolecular cellular components are removed in the Wash Step. The phenol extraction and ethanol precipitation are not required, and the high-quality nucleic acid is eluted in the RNase-free elution buffer. The viral DNA/RNA isolated with the Total Nucleic Acid Isolation Kit (Virus) is suitable for a variety of routine applications, including the Real-time PCR/RT-PCR, Automated Fluorescent DNA Sequencing, PCR, and other enzymatic reactions. The entire procedure can be completed within 15-20 minutes.
Specifications:Sample: Up to 300ul (whole blood), up to 200ul (virus sample)Format: Reagent and mini spin columnSample Types: Serum, plasma, body fluidsOperation time: 20 minutesElution volume: 50ul
Features:Isolates both viral RNA and DNA, allowing simultaneous detection of both types of virusRemoves inhibitors that might interfere with downstream assays, ensuring greater assay specificity, sensitivity and reproducibilityPrepares nucleic acid samples in only 20 minutesYields a concentrated sample that is suitable for direct assay (no precipitation required)Universal viral nucleic acid purification system – One kit for both DNA and RNA viral purification, allowing simultaneous testing of both viral typesEnvironment-friendly -Less infectious plastic waste due to the reduced number of hands-on stepsSafety – No phenol/chloroform extractionsVersatility – Spin and vacuum formats availableQuality – RNA suitable for downstream applications
Kit Components:Buffer V1 45 ml 1.5 mlBuffer V2 (Add ethanol) 6 ml (45ml)Buffer W1 45 ml 2 mlBuffer W2 (Add ethanol) 15 ml (60 ml)Buffer RE 10 ml Column VN 100 pcsCollection Tubes 100 pcs
Storage and Stability:Store at room temperature. Do not store together with acids. Stable for 6 months after receipt.
