BioGenomics™ DNA Polymerase was designed as one of enhanced hot start enzyme DNA Polymerases which provide the convenience and reliability toward your research destination. NanoTaq was engineered with nano technology complex, which is an innovative creation, different from the traditional methods of hot start enzymes made. The proven features of NanoTaq covers reactions at room temperature using the same protocol and cycling conditions as conventional Taq DNA polymerases, reducing nonspecific primer annealing, improving product yield and using for PCR products up to 5kb.
Feaures:Reactions at room temperature using the same protocol and cycling conditions as conventional Taq DNA polymerasesReduce nonspecific primer annealingImprove product yieldUse for PCR products up to 5~6kb
Unit Definition:One unit is defined as the amount of enzyme that will catalyze the incorporation of 10 nmol of dNTP into acidinsoluble form in 30 min at 74°C in a reaction containing 25 mM TAPS (tris-[hydroxymethyl]-methyl-aminopropane-sulfonic acid, sodium salt), pH 9.3 at 25°C, 50 mM KCl, 2 mM MgCl2, 1 mM β-mercaptoethanol, 0.2mM dATP, dGTP, and dTTP, 0.1uM [α-32P] dCTP, and activated salmon sperm DNA
Kit Components:NanoTaq Hot-Start DNA Polymerase, 500 units/500ul10X PCR buffer, 600ul
