Coronaviruses are species of virus belonging to the subfamily Coronavirinae in the family Coronaviridae, in the order Nidovirales. They are enveloped viruses with positive-stranded, capped, and polyadenylated RNA genomes ranging in size from 28 to 32 kb. Two-thirds of the viral genome starting from the 5’end encode replicase pro-teins, Rep1a and Rep1b, for the amplification of CoV RNA. Structural proteins, including spike (S), envelope (E), membrane (M),nucleocapsid (N), and several others with unknown functions, are also expressed (Lai et al., 1997).
They can cause severe respiratory disease and gastrointestinal illnesses in both humans and animals. During virus replication, a variety of proteins are synthesised including the viral RNA binding protein, nucleoprotein (N or nucleocapsid protein), a multi-functional protein with roles in both the virus life cycle and modulating host cell function. One of the key functions of the N protein is to bind viral RNA and selectively incorporate it into virus particles. The genome RNA is complexed with the basic nucleocapsid (N) protein to form a helical capsid found within the viral membrane. The nucleocapsid proteins of coronaviruses representing groups I, II, and III have been shown to localize to the nucleolus as well as to the cytoplasm suggesting that N protein induces a cell cycle delay or arrest, most likely in the G2/M phase, possibly by inhibition of cytokines (Weiss and Navas-Martin, 2005).
The Severe Acute Respiratory Syndrome (SARS) coronavirus was first identified in 2003 when it caused an epidemic of fatal human pneumonia cases that rapidly spread to multiple countries from an epicenter in Hong Kong. During the period of infection, there were 8,098 reported cases of SARS and 774 deaths. There’s currently no cure for SARS, and research to find a vaccine is ongoing. However, the nucleoprotein can serve as a useful target for the early diagnosis of infection.
Contains the Nucleocapsid protein immunodominant region. Immunoreactive with SARS positive sera.
Source:Recombinant protein corresponding to aa1-49 from SARS Nucleocapsid at N-terminal, strain CUHK-L2, fused to a GST-Tag expressed in E. coli.
Applications:Suitable for use in ELISA and Western Blot. Other applications not tested.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：≥95% (10% PAGE, Coomassie Staining).
Form：Supplied as a liquid in 50mM Tris-HCl, 60mM sodium chloride, 50% glycerol.
