85-6038-05　TRANCE/RANK L/TNFSF11, Recombinant, Mouse, aa158-316 50ug　532392

特徴

• Tnfsf11, also known as tumor necrosis factor ligand superfamily member 11, is a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. Tnfsf11 also has a function in the immune system, where it is expressed by T helper cells and is thought to be involved in dendritic cell maturation. Tnfsf11 is important in bone metabolism. This natural and necessary surface-bound molecule (also known as CD254) found on osteoblasts serves to activate osteoclasts, which are the cells involved in bone resorption.
• Source:Recombinant protein corresponding to aa158-316 from mouse TRANCE/RANK L/TNFSF11B, expressed in E. coli.
• Molecular Weight:~17.9 kD (160aa), confirmed by MALDI-TOF
• Endotoxin:~1EU/1ug (LAL)
• Biological Activity:Measured in a osteoclast differentiation assay using RAW 264.7 mouse monocyte/macrophage cells. The ED50 range < 2ng/ml.
• AA Sequence:MKPEAQPFAH LTINAASIPS GSHKVTLSSW YHDRGWAKIS NMTLSNGKLR VNQDGFYYLY ANICFRHHET SGSVPTDYLQ LMVYVVKTSI KIPSSHNLMK GGSTKNWSGN SEFHFYSINV GGFFKLRAGE EISIQVSNPS LLDPDQDATY FGAFKVQDID
• Applications:Suitable for use in Bioactivity and SDS-PAGE. Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.