85-6037-64　M-CSF, Recombinant, Mouse, aa33-187, His-Tag 50ug　532342

特徴

• M-CSF, also known as macrophage colony-stimulating factor 1 isoform 1, is one of the hematopoietic growth factors that regulate the growth and differentiation of blood cells. This protein is produced by monocytes, granulocytes, endothelial cells, and fibroblasts. It stimulates the formation of macrophage colonies, enhances antibody-dependent, cell-mediated cytotoxicity by monocytes and macrophages, and inhibits bone resorption byosteoclasts. It plays an essential role in the regulation of survival, proliferation and differentiation of the matopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. This protein promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and ininflammatory processes.
• Recombinant protein corresponding to aa33-187 from mouse M-CSF5 fused to His-Tag at C-terminal, expressed in Baculovirus.
• Molecular Weight:~19.1kD (164aa)
• Biological Activity:Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is < 4ng/ml.
• Amino Acid Sequence:ADPKEVSEHC SHMIGNGHLK VLQQLIDSQM ETSCQIAFEF VDQEQLDDPV CYLKKAFFLV QDIIDETMRF KDNTPNANAT ERLQELSNNL NSCFTKDYEE QNKACVRTFH ETPLQLLEKI KNFFNETKNL LEKDWNIFTK NCNNSFAKCS SRDVVTKPHH HHHH
• Applications:Suitable for use in Bioactivity and SDS-PAGE. Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.