85-6037-56　Interleukin 1 alpha, Recombinant, Rat, aa115-270, His-Tag (Il1a, IL-1F1) 100ug　532331

特徴

• B-cell maturation and proliferation, and fibroblast growth factor activity. IL1 proteins are involved in the inflammatory response, especially IL1A is an early and important mediator of inflammation after injury.
• Source:Recombinant protein corresponding to aa115-270 from rat Interleukin 1 alpha, fused to His-Tag, expressed in E. coli.
• Molecular Weight:~20.2kD (179aa) confirmed by MALDI-TOF
• Biological Activity:Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is less or equal to 15ng/ml.
• Endotoxin:<1EU/1ug (LAL)
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MGSSAPHSFQ NNLRYKLIRI VKQEFIMNDS LNQNIYVDMD RIHLKAASLN DLQLEVKFDM YAYSSGGDDSKYPVTLKVSN TQLFVSAQGE DKPVLLKEIP ETPKLITGSE TDLIFFWEKI NSKNYFTSAA FPELLIATKE QSQVHLARGL PSMIDFQIS
• Applications:Suitable for use in Bioactivity and SDS-PAGE. Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.