85-6037-33　HSP27, Recombinant, Human, aa1-209, His-Tag 100ug　532317

特徴

• HSP27, also known as heat shock protein beta-1, is one of the small heat shock proteins and constitutively expressed in various tissues. HSP27 expression is increased to high levels after various types of stress including elevated temperatures, toxic metals, drugs and oxidants. HSP27 and its phosphorylation state participate in the regulation of multiple physiological and pathophysiological cell functions. However, the exact roles of HSP27 in osteoblasts remain unclear. In the present study, we investigated the role of HSP27 in the platelet-derived growth factor-BB(PDGF-BB)-stimulated migration of osteoblast-like MC3T3-E1 cells.
• Source:Recombinant protein corresponding to aa1-209 from human HSP27, fused to His-Tag at N-terminal, expressed in E. coli.
• Molecular Weight:~25.4kD (232aa) confirmed by MALDI-TOF
• Endotoxin:~1EU/1ug (LAL)
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MGSMTERRVP FSLLRSPSWE PFRDWYPAHS RLFDQAFGVP RLPDEWSQWF SAAGWPGYVR PLPAATAEGP AAVTLAAPAF SRALNRQLSS GVSEIRQTAD RWRVSLDVNH FAPEELTVKT KEGVVEITGK HEERQDEHGY ISRCFTRKYT LPPGVDPTLV SSSLSPEGTL TVEAPLPKAV TQSAEITIPV TFEARAQIGG PEAGKSEQSG AK
• Applications:Suitable for use in SDS-PAGE. Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.