85-6036-67　Asparagine Synthetase/ASNS, Recombinant, Mouse, aa1-561, His-Tag 50ug　532260

特徴

• ASNS, also known as Asparagine synthetase, is a one of cytoplasmic enzyme that generates asparagine from aspartate. It mainly distributes in mammalian organs, but basal expression is low in tissues other than theexocrine pancreas. This protein plays a role of cell growth, and its mRNA content is linked to changes in the cell cycle. Its mRNA is upregulated in response to the asparagine depletion, some of the toxic effects of themedication on normal cell activity. Above average presence of asparagine synthetase in certain leukemia strains has been linked to be a significant contributing factor of chemotherapy resistance, particularly to the chemotherapy drug, L-asparaginase. Also, it functions as potential biomarker for ovarian cancer, potential role in solid tumor metastasis.
• Source:Recombinant protein corresponding to aa1-561 from mouse Asparagine Synthetase/ASNS fused to His-tag at C-terminal, expressed in Baculovirus..
• Molecular Weight:~65.1kD (567aa)
• Endotoxin:~1EU/1ug (LAL)
• AA Sequence:MCGIWALFGS DDCLSVQCLS AMKIAHRGPD AFRFENVNGY TNCCFGFHRL AVVDPLFGMQPIRVRKYPYL WLCYNGEIYN HKALQQRFEF EYQTNVDGEI ILHLYDKGGI EKTICMLDGVFAFILLDTAN KKVFLGRDTY GVRPLFKAMT EDGFLAVCSE AKGLVSLKHS TTPFLKVEPFLPGHYEVLDL KPNGKVASVE MVKYHHCTDE PLHAIYDSVE KLFPGFDLET VKNNLRILFDNAIKKRLMTD RRIGCLLSGG LDSSLVAASL LKQLKEAQVQ YPLQTFAIGM EDSPDLLAARKVANYIGSEH HEVLFNSEEG IQALDEVIFS LETYDITTVR ASVGMYLISK YIRKNTDSVVIFSGEGSDEL TQGYIYFHKA PSPEKAEEES ERLLKELYLF DVLRADRTTA AHGLELRVPFLDHRFSSYYL SLPPDMRIPK NGIEKHLLRE TFEDCNLLPK EILWRPKEAF SDGITSVKNSWFKILQDYVE HQVDDEMMSA SQKFPFNTP KTKEGYFYRQ IFERHYPGRA DWLTHYWMPK WINATDPSAR TLTHYKSAAK AHHHHHH
• Applications:Suitable for use in SDS-PAGE. Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.