Recombinant protein corresponding to Coronavirus, SARS Associated, Spike, fused to GST-Tag, expressed in E. coli. Contains a mosaic of the immunodominant regions of the N-terminal of the Spike protein. Not full-length spike protein. Immunoreactive with SARS positive sera.
Molecular Weight:~38kD. May appear as multiple bands on SDS-PAGE (intact protein at 38kD, GST fusion protein at 26kD and the Nucleocapsid fragment at 12kD).
Applications:Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：1mg
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：≥95% (10% PAGE, Coomassie Staining).
Form：Supplied as a liqud in 25mM Tris-HCl, 0.4% sarcosyl, 0.25% Triton-X100, 50% glycerol.
