Recombinant, Human protein for Monoclonal antibody 134743.
Human TRIM33 partial ORF (NP_056990, aa1006-1105) recombinant protein with GST-tag at N-terminal.
The protein encoded by this gene is thought to be a transcriptional corepressor. However, molecules that interact with this protein have not yet been identified. The protein is a member of the tripartite motif family. This motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Three alternatively spliced transcript variants for this gene have been described, however, the full-length nature of one variant has not been determined.
Applications:Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions:Immunofluorescence: 30ug/mlImmunohistochemistry (FFPE): 3ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Storage Buffer: 50mM Tris-HCI, 10mM reduced Glutathione, pH 8 in the elution buffer
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Stable for 3 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:10ug
Source Antigen:Recombinant, Human from Wheat Germ
Grade:Affinity Purified
Purity:Purified by Glutathione Sepharose affinity chromatography.
Form:Supplied as a liquid in 50mM Tris-HCI, 10mM reduced Glutathione, pH 8 in the elution buffer.
Specificity:Recognizes human TRIM33. Species Crossreactivity: mouse
Calc Applications Abbrev:E IF IHC WB
Calc Crossreactivity:Hu Mo
Immunogen:Partial recombinant corresponding to aa1006-1105 from human TRIM33 with GST tag. MW of the GST tag alone is 26kD.