Yersinia pestis (formerly known as Pasteurella pestis) is a Gram-negative, rod- shaped coccobacillus, which can infect humans and various animals. It causes the deadly disease called plague. Human Y. pestis infection takes three main forms: pneumonic, septicemic, and bubonic plagues. All three forms were responsible for a number of high-mortality epidemics throughout human history, including: the sixth century's Plague of Justinian and the Black Death.
The main reservoir of Y. pestis is rodents. A variety of species can be hosts, however some are known to have variable resistance which means they can be asymptomatic carriers. Y. pestis is usually transmitted to human via the bites of infected fleas. If the disease has taken the pneumonic form, then it can be transmitted by coughing and possibly sneezing. Flea bites allow for the bacteria to pass the skin barrier. Y. pestis expresses a plasminogen activator that is an important virulence factor for pneumonic plague and that might degrade on blood clots to facilitate systematic invasion. Y. pestis proliferates inside lymph nodes, where it is able to avoid destruction by cells of the immune system such as macrophages. The ability of Y. pestis to inhibit phagocytosis allows it to grow in lymph nodes and cause lymphadenopathy.
The incubation period of Y. pestis is between 2 and 6 days. Symptoms normally associated with plague are fatigue, fever and aches, gangrene and the swelling of lymph nodes. This results in buboes, the classic sign of bubonic plague. Death can occur in under two weeks. Several subspecies have been identified. Y. p. antiqua, Y. p. mediaevalis and Y. p. orientalis. The sixth-century Plague of Justinian and the Black Death are thought to have been caused by earlier, now extinct, forms of the bacterium
-Quantification of Yersinia pestis genomes. 2 genesig Standard kit handbook HB10.04.10
SPECIFICITY:The Primerdesign genesig Kit for Yersinia pestis (Y.pestis) genomes is designed for the in vitro quantification of Y.pestis genomes. The kit is designed to have a broad detection profile. Specifically, the primers represent 100% homology with over 95% of the NCBI database reference sequences available at the time of design
The dynamics of genetic variation means that new sequence information may become available after the initial design. Primerdesign periodically reviews the detection profiles of our kits and when required releases new versions.
Y.pestis specific primer/probe mix (150 reactions BROWN) FAM labelled
Y.pestis positive control template (for Standard curve RED) • RNase/DNase free water (WHITE)for resuspension of primer/probe mixes
Template preparation buffer (YELLOW)for resuspension of positive control template and standard curve preparation
REAGENTS AND EQUIPMENT TO BE SUPPLIED BY THE USER:Real-Time PCR InstrumentRNA extraction kitPipettes and Tips Vortex and Centrifuge 1.5ml tubes
This kit is recommended for use with genesig Easy DNA/RNA extraction kit. However, it is designed to work well with all processes that yield high quality RNA and DNA with minimal PCR inhibitors
oasigTM lyophilised or Precision®PLUS 2X qPCR Master MixThis kit is intended for use with oasig or PrecisionPLUS 2X qPCR Master Mix.
KIT STORAGE AND STABILITY:This kit is stable at room temperature but should be stored at --20°C on arrival. We do not recommend using the kit after the expiry date stated on the pack. Once the lyophilized components have been re-suspended, unnecessary repeated freeze/thawing should be avoided. The kit is stable for six months from the date of resuspension under these circumstances.
SUITABLE SAMPLE MATERIAL:All kinds of sample material suited for PCR amplification can be used. Please ensure the samples are suitable in terms of purity, concentration, and RNA/DNA integrity. Always run at least one negative control with the samples. To prepare a negative-control, replace the template RNA sample with RNAse/DNAse free water.
DYNAMIC RANGE OF TEST:Under optimal PCR conditions genesig Y.pestis detection kits have very high priming efficiencies of >95% and can detect less than 100 copies of target template.
INSTRUMENTATION REQUIREMENTS:Designed for use in other instruments. For use in genesig® q16, look for the “Easy” nomenclature.
ABOUT THE genesig® KITS:genesig® kits are sold for research use only and are not licensed for diagnostic procedures
The Primerdesign genesig Kit for Yersinia pestis (Y.pestis) genomes is designed for the in vitro quantification of Y.pestis genomes. The kit is designed to have a broad detection profile. Specifically, the primers represent 100% homology with over 95% of the NCBI database reference sequences available at the time of design
The dynamics of genetic variation means that new sequence information may become available after the initial design. Primerdesign periodically reviews the detection profiles of our kits and when required releases new versions
If you require further information, or have a specific question about the detection profile of this kit then please send an e.mail to [email protected] and our bioinformatics team will answer your question
-Quantification of Yersinia pestis genomes. 3 genesig Standard kit handbook HB10.04.10
DNA/RNA Extraction:genesig® Easy DNA/RNA extraction kits are a simple to use, low cost and powerful extraction technology suitable for virtually all sample types.
