85-6033-77　［取扱停止］8-epi-PGF2alpha (8-Epi-Prostaglandin F2 Alpha) BioAssay™ ELISA Kit 96Tests　529896

特徴

• This 8-epi-PGF2alpha (8-Epi-Prostaglandin F2 Alpha) BioAssay™ ELISA Kit is a competitive ELISA for the in vitro quantitative determination of 8-epi-PGF2alpha concentrations in serum, plasma and other biological fluids.
• Sensitivity:9.38pg/ml
• Detection Range:15.63-1000pg/ml
• Precision:Intra-Assay %CV: <10%Inter-Assay %CV: <10%
• Specificity:Recognizes 8-epi-PGF2alpha. No significant cross-reactivity or interference between 8-epi- PGF2alpha and analogues was observed.
• Test Principle:The micro ELISA plate provided in this kit has been pre-coated with 8-epi-PGF2alpha. During the reaction, 8-epi-PGF2alpha in the sample or standard competes with a fixed amount of 8-epi-PGF2alpha on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 8-epi-PGF2alpha. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 2nm. The concentration of 8-epi-PGF2alpha in the samples is then determined by comparing the OD of the samples to the standard curve.
• Kit Components:Micro ELISA Plate (Dismountable), 8 wells ×12 stripsReference Standard, 2 vialsConcentrated Biotinylated Detection Ab (100×), 1x120ulConcentrated HRP Conjugate (100×), 1x120ulReference Standard & Sample Diluent, 1x20mlBiotinylated Detection Ab Diluent, 1x14mlHRP Conjugate Diluent, 1x14mlConcentrated Wash Buffer (25×), 1x30mlSubstrate Reagent, 1x10mlStop Solution, 1x10ml
• Storage and Stability:Unused kit is stable for 6 months after receipt.  Store Microtiter Strips, Standard, and Antibody-Biotin at 4°C if kit will be used within 30 days; otherwise store at -20°C until expiration date. Store the rest of the components at 4°C. Protect HRP Conjugate and Substrate Reagent from light. For maximum recovery, centrifuge the original vials after thawing and prior to removing the cap.
• Assay Procedure Summary:1. Prepare all reagents, samples and standards
• 2. Add 100ul standard or sample to each well. Incubate 90 minutes at 37°C
• 3. Aspirate and add 100ul prepared Biotin Working Solution. Incubate 1 hour at 37°C
• 4. Aspirate and wash 3 times
• 5. Add 100ul prepared HRP Detection Working Solution. Incubate 30 minutes at 37°C
• 6. Aspirate and wash 5 times
• 7. Add 90ul Substrate. Incubate 15 minutes at 37°C
• 8. Add 50ul Stop Solution. Read at 450nm immediately.