Transferrin is a single polypeptide chain glycoprotein and is a member of the iron binding family of proteins. It has a molecular weight of ~79.5kD and a serum concentration range of 1800–2700mg/L. It is synthesized in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+. The iron is transported in blood and interstitial fluids to sites of use and disposal. Iron/transferrin is essential in hemoglobin synthesis and for certain types of cell division. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions.
Human Transferrin is a glycoprotein with homologous N-terminal and C-terminal iron binding domains. Transferrin(TF) is related to other iron binding proteins including lactoferrin. When human transferrin loaded with iron encounters a transferrin receptor on the surface of a cell, it binds to it and is consequently transported into the cell in a vesicle. The cell will acidify the vesicle, causing human transferrin(TF) to release its iron ions. Each human transferrin molecule has the ability to carry two iron ions in the ferric form (Fe3+).
Human Transferrin levels may be checked in iron deficiency, hemochromatosis and other iron overload disorders. Transferrin is also associated with the innate immune system. Human Transferrin is found in the mucosa and binds iron, thus creating an environment low in free iron, where few bacteria are able to survive.
Applications:Suitable for use in Cell Culture. Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Solubility (50mg/ml H2O):Light orange to dark orange, clear, complete
pH (3%):5.5-8.0
Water Content:≤10%
Iron:300-600ug/g
Heat Treatment:Heat treated at 60°C for ten hours
Storage and Stability:Lyophilized powder may be stored at -20°C. Sable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Toxicity and Hazards:All products should be handled by qualified personnel only, trained in laboratory procedures.
