The TP53 Antibody (p53 Antibody) BioAssay™ ELISA Kit (Human) is a quantitative sandwich assay for the detection of TP53 antibody in human serum, plasma and other biological fluids. Cell lysates and tissue homogenates, though not tested, may potentially be used as samples.
Sensitivity:0.469ng/ml
Detection Range:0.781-50ng/ml
Specificity:This assay has high sensitivity and excellent specificity for detection of human TP53 antibody. No significant cross-reactivity or interference between TP53 antibody and analogues was observed.
Precision:Intra-Assay: CV<8%Inter-Assay: CV<10%
Test Principle:This ELISA kit employs the sandwich enzyme-linked immune-sorbent assay technology. TP53 was pre-coated onto 96-well plates. The Biotin-labeled TP53 was used as detection antigen. The standards, test samples and Biotin-labeled TP53 were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin Conjugate was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellowafter adding acidic stop solution. The density of yellow is proportional to the TP53 antibody amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TP53 antibody can be calculated.
Storage and Stability:Store unopened kit at 4ºC; store at -20ºC once opened. Store unopened standard at 4°C; once reconstituted, store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
