The Spermine BioAssay™ ELISA Kit is a quantitative competitive assay for the detection of Spermine in serum, plasma, tissue homogenates and other biological fluids.
Sensitivity:7.5ng/ml
Detection Range:12.5-800ng/ml
Specificity:This assay has high sensitivity and excellent specificity for detection of Spermine. No significant cross-reactivity or interference between Spermine and analogues was observed.
Precision:Intra-Assay: CV<8%Inter-Assay: CV<10%
Test Principle:This BioAssay™ ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this BioAssay™ kit has been pre-coated with Spermine. During the reaction, Spermine in the sample or standardcompetes with a fixed amount of Spermine on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to Spermine. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of Spermine in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Storage and Stability:Store unopened kit at 4ºC; store at -20ºC once opened. Store unopened standard at 4°C; once reconstituted, store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
