The GLI1 (Zinc Finger Protein GLI1) BioAssay™ ELISA Kit (Rat) is a quantitative sandwich assay for the detection of GLI1 in serum, plasma, tissue homogenates and other biological fluids.
Sensitivity:0.094ng/ml
Detection Range:0.156-10ng/ml
Specificity:This assay has high sensitivity and excellent specificity for detection of rat GLI1. No significant cross-reactivity or interference between GLI1 and analogues was observed.
Precision:Intra-Assay: CV<8%Inter-Assay: CV<10%
Test Principle:This ELISA kit employs the sandwich enzyme-linked immune-sorbent assay technology. Anti-GLI1 antibody was pre-coated onto 96-well plates. The biotin conjugated anti-GLI1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP toproduce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the GLI1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of GLI1 can be calculated.
Storage and Stability:Store unopened kit at 4ºC; store at -20ºC once opened. Store unopened standard at 4°C; once reconstituted, store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
