The Biotin BioAssay™ ELISA Kit is a quantitative competitive assay for the detection of Biotin in serum, plasma, tissue homogenates and other biological fluids.
Sensitivity:15ng/ml
Detection Range:25-1600ng/ml
Specificity:This assay has high sensitivity and excellent specificity for detection of Biotin. No significant cross-reactivity or interference between Biotin and analogues is observed.
Precision:Intra-Assay: CV<8%Inter-Assay: CV<10%
Test Principle:The microtiter plate provided in this kit has been pre-coated with SA. During the reaction, Biotin in the sample or standard competes with a fixed amount of HRP-biotin. Excess conjugate and unbound sample or standard are washed from the plate. Then TMB substrate solution is added to each well. The enzymesubstrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of Biotin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Storage and Stability:Store unopened kit at 4ºC; store at -20ºC once opened. Store unopened standard at 4°C; once reconstituted, store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
