85-6028-59　［取扱停止］Insulin, Recombinant, Human (S. cerevisiae) 10mg　CAS No:11070-73-8　519064

特徴

• Recombinant Human Insulin is commonly used in the growth of mammalian cell lines. The recombinant form is identical to the native human sequence in function and structure. Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
• Insulin Human Recombinant produced in Yeast is a two chain, glycosylated polypeptide chain containing 51 amino acids and having a molecular mass of 5807D. Zinc content was found to be 0.4%. Insulin is purified by proprietary chromatographic techniques.
• Source:Saccharomyces cerevisiae.
• Purity:≥95% by SDS-PAGE
• Appearance:Sterile-filtered, white lyophilized powder from 50mM sodium chloride solution. Reconstitute in PBS pH-7.5.
• Biological Activity:27.5 units/mg. Insulin has been evaluated in cell culture (Human Foreskin Fibroblasts). The effective concentration range for use in defined media is 1-5ug/ml. However, the optimum concentration range for a particular application must be determined by the investigator. 
• Storage:Stable at RT for short-term Store at 4°C. Upon reconstitution Insulin should be stored at -20°C to -80°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Prevent freeze-thaw cycles.