Zika virus (ZIKV) is a member of the virus family Flaviviridae and the genusFlavivirus, transmitted by Aedes mosquitoes, such as A. aegypti and A
albopictus. Its name comes from the Zika Forest of Uganda, where the virus was first isolated in 1947. Zika virus is related to Dengue virus, Yellow Fever, Japanese Encephalitis, and West Nile viruses, and antibodies (e.g. those produced as a response to infection) may cross-react between these viruses. The viral genome is expressed as a single large polypeptide, and NS1 is an early marker of viral replication. It has been reported in human serum (Bosch et al Sci Transl Med. 9 (409) (2017)).
Sample Type:Serum, urine and culture medium
Intended Use:This Zika Virus NS1 Capture ELISA BioAssay™ kit is designed for the quantitative measurement of Zika virus NS1 (ZIKV NS1) in whole blood, serum/plasma, urine and culture fluids.
Sensitivity:5pg/ml
Test Principle:The NAC ZIKV NS1 Capture ELISA uses two specific monoclonal antibodies to capture and detect ZIKV NS1. These antibodies are specific for ZIKV NS1, and do not cross-react with Dengue virus, WNV, JEV, YFV, Usutu or TBEV NS1. Samples containing the target analyte are pre-diluted in a diluent designed to reduce interfering factors and enhance binding. The diluted samples are then incubated in microtiter plate wells coated with stabilized capture antibody. After incubation and washing, a second specific antibody coupled to biotin is added to detect the bound NS1, and then subsequently streptavidin-HRP is used to probe the biotinylated antibody. Finally TMB is added to give a colored product in proportion to the amount of HRP bound and the reaction stopped with acid solution. Absorbance of the wells is measured at 450nm and a reference reading may also be taken between 620-650nm. Standards supplied with the kit are used to plot a standard curve of O.D. vs. concentration, from which the concentrations of the samples may be calculated.
Kit Components:96 well microtiter plate with stabilized anti-Zika virus NS1 coated wellsKit standards, 5xbottles, lyophilizedZero standard (BLUE), 1x30ml including 0.5% Proclin 950Wash Buffer 25X, 1xbottle, 100mlAntibody-biotin conjugate (PINK) ready to use, 1x12ml including 0.5% Proclin 950Streptavidin-HRP conjugate ready to use, 1x12ml including 0.5% Proclin 950TMB ready to use, 1x12ml1M Hydrochloric Acid, 1x12mlSelf-adhesive sealing film, x6
Storage and Stability:Store the un-opened kit at 2-8°C. After reconstitution in zero standard the kit standards must be stored at -20°C.
