Yellow fever is an acute hemorrhagic disease caused by the yellow fever virus (YFV), which is a member of the Flaviviridae family of viruses. Clinical symptoms of the disease include fever, muscle pain, nausea and vomiting. In a small percentage of patients, the liver and kidneys are affected leading to jaundice, and in some cases death. Yellow fever virus is endemic in tropical areas of Africa and Central/South America where the vector is widespread. In the sylvatic cycle, the virus is transmitted to non-human primates via mosquitoes of the Haemagogus and Sabethes genera, with occasional outbreaks of Yellow Fever in individuals working in forest areas. Whereas the Aedes aegypti mosquito is responsible for the transmission of Yellow Fever virus to humans in urban areas. In the late 1930’s a safe and effective attenuated vaccine was developed against the YFV, which confers long-term immunity (Norrby, E). The virulent wild-type Asibi strain of YFV was used to develop the YFV 17D vaccine, which is used successfully to immunize individuals at risk of YFV infection (WHO).
Source:Yellow Fever virus, strain Asibi, expressed in the Vero cell line
Applications:Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Inactivation:Purified virions were lysed in 0.6M KCl containing 0.5% Triton X-100, and then subjected to heat inactivation. Verified by the absence of viral growth in validated cell culture based infectivityassays.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Source Antigen：Yellow Fever virus, strain Asibi, expressed in the Vero cell line
Grade：Purified
Purity：Purified by sucrose density gradient ultracentrifugation
