West Nile virus (WNV) is an enveloped, single-stranded RNA virus that belongs to the genus Flavivirus. It is a member of the Japanese encephalitis sero-complex of the family Flaviviridae, which also includes Japanese encephalitis virus, St. Louis encephalitis and Murray Valley encephalitis virus. In nature, West Nile virus is maintained in a cycle between birds and mosquitoes. However, WNV can also be transmitted to incidental hosts such as humans, horses and other mammals by mosquito vectors (Chancey, C). Since the first human WNV infection was identified in 1937 the virus has continued to spread. West Nile virus is now found in many regions of the world including Africa, Europe, West Asia, North and South America. West Nile virus infection is asymptomatic in 80% of cases but some individuals develop severe West Nile fever. The symptoms of West Nile fever include fever, headache, nausea and vomiting. The virus has also been associated with neurological complications and fatalities in less than 1% of infected individuals.
Source:West Nile Virus, lineage 2 (1986 strain), expressed in Vero cell line
Applications:Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Inactivation:Purified virions were lysed in 0.6M KCl containing 0.5% Triton X-100, and then subjected to heat inactivation. Verified by the absence of viral growth in validated cell culture based infectivity assays.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Source Antigen：West Nile Virus, lineage 2 (1986 strain), expressed in Vero cell line
Grade：Purified
Purity：Purified by sucrose density gradient ultracentrifugation
