Saint Louis encephalitis is a disease caused by the mosquito borne Saint Louis encephalitis virus (SLEV). SLEV is within the Japanese encephalitis virus (JEV) complex along with other flaviviruses including Murray Valley encephalitis virus (MVEV) and West Nile virus (WNV).
The SLEV genome is ~11kb, single-stranded, positive-sense RNA and encodes a polyprotein flanked by 5′ and 3′ untranslated regions. At the nucleotide and amino acid level, SLEV has much lower variation than other members of the Japanese encephalitis virus complex. The polyprotein is co- and post-translationally processed into the three structural proteins in the order, capsid (C), pre-membrane/membrane (prM/M) and envelope (E) and seven non-structural proteins: NS1, NS2A, NS2B, NS3, NS4A, NS4B and NS5. NS1 involved in immune evasion, pathogenesis and viral replication. Following cleavage, it is targeted to three destinations; the viral replication cycle, the plasma membrane and the extracellular compartment. It is essential for viral replication where it is required for formation of the replication complex and recruitment of other non-structural proteins to the ER-derived membrane structures. It is also excreted as a hexameric lipoparticle that plays a role against host immune response. It binds to the host macrophages and dendritic cells, antagonizing the complement function and inhibiting signal transduction originating from Toll-like receptor 3 (TLR3).
Recombinant protein corresponding to St Louis Encephalitis NS1, strain MS1-7, fused to His-Tag at C-terminal, expressed in HEK293 cells.
Molecular Weight:~25kD
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
