Rubella virus is an enveloped, positive single-stranded RNA virus and a member of the genus Rubivirus, which belongs to the Togaviridae family. The structural proteins of Rubella virus are synthesized as a polyprotein precursor in association with the endoplasmic reticulum in the host cell. It consists of three structural proteins: a capsid protein and two membrane-spanning glycoproteins, E1 and E2, localized in the virus envelope (Oker-Blom, et al., 1983). The polyprotein is cotranslationally cleaved by the host-cell signal peptidase and the C-terminal of the capsid protein remains attached to the E2 signal peptide, which anchors this viral protein to intracellular membranes (Suomalainen, et al., 1990). The capsid protein, excluding the E2 signal peptide, is a 277aa residue protein that forms disulfide-linked dimers (Baron & Forsell, 1991). It interacts with the RNA genome and forms the nucleocapsid, which is surrounded by a lipid membrane upon which E1 and E2 are arranged (Hobman & Chantler, 2007). The capsid protein is an essential component of the virus and a key factor for successful replication of the virus in host cells (Mangala Prasad, et al., 2013). It not only packages the RNA genome but also is involved in viral transcription and replication, regulating viral RNA replication by interacting with the virus-encoded nonstructural proteins (Chen & Icenogle, 2004).
Recombinant protein corresponding to aa1-300 of strain F-Therien of Rubella virus capsid Nucleoprotein, fused to C-terminal 10aa glycine-serine linker followed by a 6xHis-Tag, expressed in HEK293 cells.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Source Antigen：Recombinant, HEK293 cells
Grade：Highly Purified
Purity：≥95%
Form：Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 50mM sodium chloride, 0.5% SDS.
