85-6028-16　［取扱停止］Neuraminidase (NA), Recombinant, Influenza A [A/Thailand/1(KAN-1)/2004 (H5N1)], aa36-449, His-Tag (H5N1) 100ug　519024

特徴

• Influenza, commonly known as “the flu”, is an infectious disease of birds and mammals caused by RNA viruses of the family Orthomyxoviridae, the influenza viruses. The virus is divided into three main types (Influenza virus A, Influenza virus B, and Influenza virus C), which are distinguished by differences in two major internal proteins (hemagglutinin (HA) and neuraminidase (NA), which are the most important targets for the immune system. The type A viruses are the most virulent human pathogens among the three influenza types and cause the most severe disease. Influenza virus neuraminidase (NA) is a mushroom-shaped tetramer of identical subunits. Each of the subunits that form the head of the mushroom is made up of a propeller-like structure, the blades of which are formed by four antiparallel strands of beta-structure. The enzyme active site is located at roughly the center of each subunit. During virus replication, Influenza virus neuraminidase removes sialic acid from cellular glycoproteins and glycolipids and from both of the virus glycoproteins. As a result, newly assembled viruses are prevented from binding to the infected cell surface and from aggregating with each other through HA-sialic acid interactions. Instead, they are released from the cell to infect new cells and spread the infection. The structure of NA has been reviewed by Gamblin and Skehel in 2010.
• Recombinant protein corresponding to His36-Lys449 of Influenza A [A/Thailand/1(KAN-1)/2004 (H5N1)] Neuraminidase (NA), fused to His-Tag at C-terminal, expressed in HEK293 cells.
• Molecular Weight:~46.1kD (calculated), reduced protein migrates as a band of ~48kD
• Purity:≥90% (SDS-PAGE)
• Form:Supplied as a lyophilized powder from DPBS, pH 7.4. Reconstitute with 1ml sterile ddH2O. Solubilize for 30 to 60 minutes at RT with occasional gentle mixing.
• Storage and Stability:Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.