The 5′ end of pre-S1, pre-S2 and S regions all have independent start codons which can each generate three co-terminal envelope proteins called large (L; pre-S1+pre-S2+S), middle (M; pre-S2+S), and small (S; with S domain alone) envelope proteins. The S protein is the most abundant envelope protein expressed and can be secreted independently of L and M envelope proteins. After secretion, mature HBeAg is deleted at residue 149 C-terminal and retains 10 pre-core residues N-terminal. Both L and S envelope proteins are needed for virion secretion, while M protein is dispensable (Bruss & Ganem, 1991; Ueda et al., 1991). Core particles with mature (double stranded DNA) genome can be enveloped and released as 42nm virions, with the three envelope proteins inserted on the surface. Empty viral particles (22nm) are also abundantly produced (Bruss & Ganem, 1991; Ganem & Prince, 2004; Zhang & Tang, 2017). HBcAg is one of the three major clinical antigens of hepatitis B virus but disappears early in the course of infection. It is a highly immunogenic subviral particle comprising 180 or 240 copies of the core protein and functions as both a T-cell-dependent and a T-cell-independent antigen.
Immunoreactive with serum of HBV infected individuals.
Recombinant protein corresponding to Hepatitis B Core, comprising the HBV core immunodominant region, expressed in E. coli.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：≥95% (SDS-PAGE)
Form：Supplied as a liquid in 10mM Tris-HCl, pH 8.0, 50mM sodium chloride, 1mM EDTA, 50% glycerol.
