Puumala virus (PUUV) is an enveloped, single-stranded negative-sense RNA virus. PUUV is a Hantavirus that belongs to the Hantavirus genus, within the family Bunyaviridae. Hantaviruses are zoonotic pathogens that have global distribution. Rodents and small mammals act as natural reservoirs for Hantaviruses, with each Hantavirus strain being transmitted by a different rodent host species or related species. The hantavirus virion comprises three genome segments S, M and L, that encode the nucleocapsid (N) protein, glycoprotein precursor (GPC) and RNA-dependent RNA-polymerase, respectively (Schmaljohn, 2007). GPC is a polyprotein of aa1133–1158 residues in length (Spiropoulou, 2011). Cotranslational cleavage of GPC (aa1133–1158 polyprotein) at a site C-terminal to a conserved ‘WAASA’ sequence by the cellular signal peptidase complex produces glycoproteins G1/Gn and G2/Gc, respectively (Löber, et al., 2001). These glycoproteins are responsible for virus attachment and entry into the host cells and are considered to be a major determinant of virus pathogenicity (Spiropoulou, 2011). The G1 and G2 cytoplasmic tails interact with virus encoded ribonucleoprotein (RNP) complexes and are involved in virion assembly (Schmaljohn, et al., 1985; Hepojoki, et al., 2012). They form a spike complex, which is located on the outer surface of the virion with each spike containing four molecules of both glycoproteins. Virus-membrane fusion activity has been associated with G2 through the identification of a fusion peptide which appears to be conserved across the Bunyaviridae (Battisti, et al., 2011). Chimeric VLPs have been described for the generation of virus-reactive mAbs against hantavirus G2 glycoprotein (Zvirbliene, et al., 2014) which will be a useful tool for the development of diagnostic assays.
Recombinant protein corresponding to aa646-1116 of Puumala virus Glycoprotein 2 (Gc), strain Umea/hu, fused to C-terminal using a 15aa glycine-serine linker and a human IgG1 Fc-tag, expressed in HEK293 cells.
Molecular Weight:~25kD
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Source Antigen：Recombinant, HEK293 cells
Grade：Purified
Purity：≥90%
Form：Supplied as a liquid in DPBS, pH 7.4. Contains traces of CHAPS.
