ErpD (OspE/F-Related Protein D), also known as Arp37, is encoded by the spirochete B. burgdorferi, which is carried by Ixodes ticks. Strain B31 is the type strain (ATCC 35210) for this organism and was derived by limited dilutional cloning from the original Lyme-disease tick isolate derived by A. Barbour (Johnson, et al., 1984). Several studies have demonstrated that infected humans and animals produce antibodies directed against Erp proteins within the first 2-4 weeks of infection, suggesting Erp synthesis occurs during the initial stages of vertebrate infection. Surface-exposed Erp proteins may then facilitate interactions with host tissues during the establishment of vertebrate infection (Fraser, et al., 1997). Erp proteins from B. burgdorferi are believed to be lipoproteins, based on their predicted amino acid sequences. The spirochete migrates from the tick midgut during feeding to its salivary glands and are thus transmitted to the mammal host. This transition may be facilitated by changes in expression of some B. burgdorferi genes. It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH, and nutrients) and/or in coordination with the course of infection of the mammal host (Stevenson, et al., 1996).
Recombinant protein corresponding to Borrelia burgdorferi Erpd/Arp37, strain B31, fused to MBP-Tag, expressed in E. coli.
Molecular Weight:~78kD
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Source Antigen：Recombinant, E. coli
Grade：Purified
Purity：~90% (SDS-PAGE)
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
